De nombreuses entreprises ont mis au point un test rapide de dépistage immunologique du Covid-19 à partir d'un échantillon de sang.
Principe⚓
Le test 2019-nCoV IgG/IgM est une méthode d'immunochromatographie sur phase solide.
Complément : Extrait de la fiche technique
PRINCIPLE OF DETECTION
This product is based on capture and solid-phase immunochromatography methods for determination.
The specimen (whole blood / serum / plasma) flows from the blood separator through to the conjugate release pad (which occurs the conjugation reaction between IgG / IgM antibody in the specimen and the antigen colloidal gold of 2019-nCoV to form an immune complex of IgG I IgM antibody and colloidal gold-labeled antigen) due to capillary action.
Then migrate to a capture zone of nitrocellulose membrane-immobilized antibody (mouse-anti-human IgM antibody ) to form an immune complex of colloidal gold-labeled antigen, IgM antibody and mouse-anti-human IgM antibody, thereby generating a IgM red line.
The unreacted immune complex continues to flow upward, will be captured by the mouse-anti-human IgG antibodies to form an immune complex of colloidal gold-labeled antigen, IgG antibody and mouse-anti-human IgG antibody, thereby generating a IgG red line.
The remaining uncaptured immune complex moves upward, combining with C line (quality control line) to indicate the completion of this reaction.
Lecture⚓
Le diagramme ci-dessous présente l'évolution des anticorps dans le sérum d'un patient atteint de Covid-19.
Les résultats de 4 patients sont présentés ci-dessous :
